THE TRANSFUSION OF H$sup 3$-THYMIDINE LABELED HOMOLOGOUS BONE MARROW INTO TOTAL BODY IRRADIATED RATS (in German)
Tritium labeled thi-midine was incorporated in vitro into bone marrow cells during DNA-synthesis thus indicating their proliferative potentials. Thymidine labeled bone marrow cells were transfused into total body- irradiated rats immediately after gamma irradiation (Co/sup 60/). The labeled cells were traced in marrow, spleen, and lymphnode smears by means of high resolution autoradiography. The recipient, irradiated rats were sacrificed 1. 14. and 38 hr after transfusion of the labeled cell suspension. A fraction of the labeled cells was found in bone marrow, spleen, lymphnode. and blood of the irradiated recipient. One hour after injection a fraction of labeled immature cells was still present in the peripheral blood. After 38 hr some labeled segmented neutrophiles were found in the peripheral blood; this indicated proliferation and maturation of transfused, labeled precursor forms. It was concluded that transfused immature bone marrow cells were able to divide and mature in radiation damaged organs. The small number of labeled cells per total number of marrow cells in the recipient bone marrow suggested that the proliferative potential of transfused bone marrow cells into irradiated recipients may not be the most important factor for the stimulation of the bone marrow recovery seen after marrow cell transfusions. (auth)
- Research Organization:
- Universitat, Heidelberg, Ger.
- NSA Number:
- NSA-14-025189
- OSTI ID:
- 4148166
- Journal Information:
- Nuclear-Med., Journal Name: Nuclear-Med. Vol. Vol: 1
- Country of Publication:
- Country unknown/Code not available
- Language:
- German
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