STABILITY OF RNA AND DNA IN BONE MARROW CELLS, STUDIED WITH TRITIATED CYTIDINE AND THYMIDINE
DNA and RNA metabolism was studied in the bone marrow of the rat, using tritiated thymidine (H/sup 3/Th), a specific precursor for DNA, and tritiated cytidine (H/sup 3/C), a common precursor for both RNA and DNA. With H/sup 3/C, differential incorporation into RNA, DNA, or the soluble pool at different times after administration of the precursor was determined autoradiographically in the single cell, and/or chemically for cell populations by means of differential extraction using appropriate treatment with perchloric acid. After the initial several hours, the curves for macromolecular RNA, DNA, and acid soluble RNA were found in general to be parallel in animals given H/sup 3/C, and parallel to curves for tritium label in DNA following H/sup 3/Th administration. The expected rate of falloff in label, from kinetics of the rat bone marrow cell populations studied separately by H/sup 3/Th and autoradiography, assuming no turnover of RNA or DNA and loss of label only by loss of marrow cells by division and maturation, was in agreement with the slopes obtained. The results indicate that soluble and macromolecular RNA, once they are synthesized, are retained by the bone marrow cell in a manner indistinguishable from that for DNA. Data on RNA and DNA precursor relationships, and evidence for a late precursor for DNA following H/sup 3/C administration are presented. (auth)
- Research Organization:
- Brookhaven National Lab., Upton, N.Y.
- NSA Number:
- NSA-15-017903
- OSTI ID:
- 4019152
- Report Number(s):
- BNL-5335
- Country of Publication:
- United States
- Language:
- English
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