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OBSERVATIONS ON NUCLEAR RNA LABELED WITH TRITIATED CYTIDINE IN HUMAN CANCER CELLS IN CULTURE (HeLa)

Technical Report ·
OSTI ID:4038013
The total H/sup 3/ activity of the RNA fraction of HeLa tissue culture cells was measured at varying times after 90 and 120 min exposure of the cultures to H/sup 3/-cytidine. The results indicate that (1) the majority of macromolecular RNA is preserved, (2) some of the initially labeled RNA converted temporarily to a soluble fraction from which it was recovered as macromolecular RNA within 4 to 6 hr, and(3) the majority of newly synthesized macromolecular RNA in the nucleus dissociated from the chromatin during mitosis and contributed to cytoplasmic RNA. (D.L.C.)
Research Organization:
Brookhaven National Lab., Upton, N.Y.
NSA Number:
NSA-15-023186
OSTI ID:
4038013
Report Number(s):
BNL-5417
Country of Publication:
United States
Language:
English

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