Methylation of nucleolar RNA in HeLa cells studied by autoradiography

Journal Article · Sun Jan 01 04:00:00 EST 1984 · J. Ultrastruct. Res.; (United States)

DOI:https://doi.org/10.1016/S0022-5320(84)90094-7· OSTI ID:6350145

Cervera, J; Martinez, A; Renau-Piqueras, J

Methylation of nucleolar RNA was studied by autoradiography in HeLa cells using L-(methyl-/sup 3/H)methionine and S-adenosyl-L-(methyl-/sup 3/H)methionine as radioactive precursors. Pulse-labeling experiments show that nucleolar RNA methylation occurs on the newly synthesized RNA at the nucleolar fibrillar RNP component and mostly on the fibrillar ring of fibrillar centers, where pre-rRNA is being synthesized. Pulse-chase experiments show a shift of silver grains from the nucleolar fibrillar RNP component to the nucleolar granular component first and then to the cytoplasm. Labeling of nucleolar RNA via specific methylation permits the study of intranucleolar processing of pre-rRNA and confirms the sequence of labeling of the two nucleolar RNP components observed with radioactive uridine.

• Research Organization: Instituto de Investigaciones Citologicas, Caja de Ahorros de Valencia, Spain
• OSTI ID: 6350145
• Journal Information: J. Ultrastruct. Res.; (United States), Journal Name: J. Ultrastruct. Res.; (United States) Vol. 86:1; ISSN JULRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AUTORADIOGRAPHY
BIOLOGY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
DRUGS
HELA CELLS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
METHIONINE
METHYLATION
NUCLEIC ACIDS
NUCLEOLI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIOBIOLOGY
RNA
TRITIUM COMPOUNDS