STUDIES ON LIPID BIOSYNTHESIS IN THE ISOLATED LIVER OF THE RAT: EFFECT OF PERFUSION OF BLOOD OF IRRADIATED ANIMALS
Formation of lipids in isolated liver was studied by their incorporation of acetate-1-C/sup 14/ during perfusion of the liver yvith heparinized blood of normal and irradiated rats. Blood was obtained 24 hr after irradiation with a single dose of 3500 r. Synthesis of both cholesterol and fatty acids was significantiy stimulated by blood of irradiated animals. The mean specific activities with normal and irradiated blood, respectively, were 1185 and 2290 cpm/ mg for cholesterol, and 387 and 589 for fatty acids. The incorporation of C/sup 14/ into lipids of the perfusing serum was also greater 4 hr later with lrradiated blood; the respective values of specific activity were 421 and 1110 cpm/mg for cholesterol, and 65 and 138 fatty acids. The results are in accord with previous findings that irradiation of rats causes accumulation of lipid in the liver in the intact animal. (H.H.D.)
- Research Organization:
- Universita, Milan
- NSA Number:
- NSA-16-032845
- OSTI ID:
- 4779508
- Journal Information:
- Atti accad. med. lombarda, Journal Name: Atti accad. med. lombarda Vol. Vol: 16
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
THE EFFECT OF X-IRRADIATION AND GLUCOSE ON BRAIN LIPID SYNTHESIS
THE SYNTHESIS OF CHYLOMICRA AND MAINTENANCE OF NORMAL BLOOD SUGAR LEVELS BY ISOLATED, PERFUSED RAT LIVER