THE EFFECT OF TEMPERATURE ON THE CROSS-LINKING OF DEOXYRIBONUCLEIC ACID STRANDS BY ULTRAVIOLET RADIATION

Journal Article · Mon Sep 17 00:00:00 EDT 1962 · Biochim. et Biophys. Acta.

DOI:https://doi.org/10.1016/0926-6550(62)90150-0· OSTI ID:4778445

Glisin, V R; Doty, P

The effect of temperature of ultraviolet radiation on the cross-linking of desoxyribonucleic acid (DNA) in Salmonella typhimurium was measured at two different ionic strengths. Each sample was preheated to the desired temperature and exposed to a dose of 17,500 ergs/mm. The samples were then denatured in formamide, dialyzed against 0.15 M NaCl and bonded in a CsCl gradient in an analytical centrifuge. Films of all samples were traced with a microdensitometer and the areas representing the crosslinked and uncrosslinked DNA were calculated. Data showing the biological and physical heterogeneity of denatured ultraviolet- irradiated DNA are presented graphically. (C.H.)

• Research Organization: Harvard Univ., Cambridge, Mass.
• NSA Number: NSA-16-031482
• OSTI ID: 4778445
• Journal Information: Biochim. et Biophys. Acta., Journal Name: Biochim. et Biophys. Acta. Vol. Vol: 61
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AMIDES
BACTERIA
BIOCHEMISTRY
BIOLOGY AND MEDICINE
CENTRIFUGATION
FORMAMIDE
FORMIC ACID
MATHEMATICS
NUCLEIC ACIDS
RADIATION DOSES
RADIATION EFFECTS
SALMONELLA
TEMPERATURE
ULTRAVIOLET RADIATION