ULTRAVIOLET LIGHT INDUCED LINKING OF DEOXYRIBONUCLEIC ACID STRANDS AND ITS REVERSAL BY PHOTOREACTIVATING ENZYME

Journal Article · Thu Jun 01 04:00:00 EDT 1961 · Proc. Natl. Acad. Sci. U.S.

DOI:https://doi.org/10.1073/pnas.47.6.778· OSTI ID:4068056

Marmur, J; Grossman, L

The irradiation of DNA in solution with ultraviolet light with doses approximating those used for the inactivation of transforming activity causes the irreversible hydrogenbond breakage, most likely in internal regions of the DNA molecule and the formation of formamide- and heat-stable linkages between the two complementary DNA strands. Repair of the latter damage is catalyzed by the photoreactivating enzyme from bakers' yeast. The cross-links are in keeping with the observations of others on the irradiation of thymine in the frozen state and may be due to the formation of dimers of thymine. (auth)

• Research Organization: Brandeis Univ., Waltham, Mass.
• Sponsoring Organization: USDOE
• NSA Number: NSA-15-022122
• OSTI ID: 4068056
• Journal Information: Proc. Natl. Acad. Sci. U.S., Journal Name: Proc. Natl. Acad. Sci. U.S. Vol. Vol: 47
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AMINO ACIDS
BIOLOGY AND MEDICINE
ENZYMES
NUCLEIC ACIDS
SOLUTIONS
ULTRAVIOLET RADIATION
YEASTS