Photoreactivating enzyme from Escherichia coli

Journal Article · Wed Oct 01 04:00:00 EDT 1980 · Biophys. J.; (United States)

DOI:https://doi.org/10.1016/S0006-3495(80)84948-4· OSTI ID:6856978

Sutherlan, J C; Sutherland, B M; Cimino, G D; Griffin, K P

In photoreactivation, photochemical damage produced in DNA by far ultraviolet radiation (lambda < 320 nm) is repaired in an enzyme-mediated reaction using longer wavelength light (310 < lambda < 450 nm for E. coli). Photoreactivating enzyme (PRE) acts on a single class of photoproducts, cyclobutyl pyrimidine dimers, in an otherwise normal DNA strand at least nine bases long. PRE is one of the few DNA repair enzymes which has been purified to homogeneity in quantities sufficient for physico-chemical studies.

• Research Organization: Brookhaven National Lab., Upton, NY
• OSTI ID: 6856978
• Journal Information: Biophys. J.; (United States), Journal Name: Biophys. J.; (United States) Vol. 32:1; ISSN BIOJA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AZINES
BACTERIA
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DIMERS
DNA
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
HETEROCYCLIC COMPOUNDS
MICROORGANISMS
MOLECULAR STRUCTURE
MORPHOLOGICAL CHANGES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOREACTIVATION
PYRIMIDINES
RADIATIONS
RECOVERY
REPAIR
STRUCTURAL CHEMICAL ANALYSIS
ULTRASTRUCTURAL CHANGES
ULTRAVIOLET RADIATION