Photoreactivation of pyrimidine dimers in the DNA of normal and xeroderma pigmentosum cells

Journal Article · Tue Jan 27 04:00:00 EST 1976 · Biochemistry, v. 15, no. 2, pp. 402-406

DOI:https://doi.org/10.1021/bi00647a025· OSTI ID:4106279

Sutherland, B M; Oliver, R; Fuselier, C O; Sutherland, J C

Photoproducts formed in the DNA of human cells irradiated with ultraviolet light (uv) were identified as cyclobutyl pyrimidine dimers by their chromatographic mobility, reversibility to monomers upon short wavelength uv irradiation, and comparison of the kinetics of this monomerization with that of authentic cis--syn thymine--thymine dimers prepared by irradiation of thymine in ice. The level of cellular photoreactivation of these dimers reflects the level of photoreactivating enzyme measured in cell extracts. Action spectra for cellular dimer photoreactivation in the xeroderma pigmentosum line XP12BE agree in range (300 nm to at least 577 nm) and maximum (near 400 nm) with that for photoreactivation by purified human photoreactivating enzyme. Normal human cells can also photoreactivate dimers in their DNA. The action spectrum for the cellular monomerization of dimers is similar to that for photoreactivation by the photoreactivating enzyme in extracts of normal human fibroblasts. (auth)

• Research Organization: Univ. of California, Irvine
• Sponsoring Organization: USDOE
• NSA Number: NSA-33-017304
• OSTI ID: 4106279
• Journal Information: Biochemistry, v. 15, no. 2, pp. 402-406, Journal Name: Biochemistry, v. 15, no. 2, pp. 402-406; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

*ANIMAL CELLS-- BIOLOGICAL RADIATION EFFECTS
*DIMERS-- BIOLOGICAL RADIATION EFFECTS
*DNA-- BIOLOGICAL RADIATION EFFECTS
*PYRIMIDINES-- PHOTOREACTIVATION
560111* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Biochemicals--In Vitro
MONOMERS
N48110* --Life Sciences--Radiation Effects on Biochemicals-- In-Vitro
SPECTRA
ULTRAVIOLET RADIATION