DENATURATION OF DEOXYRIBONUCLEIC ACID OF ESCHERICHIA COLI BY X-IRRADIATION IN VITRO
Desoxyribonucleic acid (DNA) extracted from Escherichia coli was examined for the effects of x radiation on the ultraviolet absorption spectrum. Typical ultraviolet absorption curves obtained from nonirradiated, irradiated, and acid-denatured DNA solutions are presented. A comparison of the curves shows that the denaturation factor increased rapidly with dose, to about 541% of the maximum possible denaturation abserved in the acid denatured control. It is concluded that there is a progressive increase tn the disruption of hydrogen bonds as determined from the increase in the extinction coefficient with increasing radiation dose. (C.H.)
- Research Organization:
- Saha Inst. of Nuclear Physics, Calcutta
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-17-005855
- OSTI ID:
- 4750853
- Journal Information:
- Nature, Journal Name: Nature Vol. Vol: 196
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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