GENETIC TRANSFORMATION STUDIES. III. EFFECT OF ULTRAVIOLET LIGHT ON THE MOLECULAR PROPERTIES OF NORMAL AND HALOGENATED DEOXYRIBONUCLEIC ACID

Journal Article · Tue Jun 25 04:00:00 EDT 1963 · Biochimica et Biophysica Acta (Netherlands)

DOI:https://doi.org/10.1016/0006-3002(63)90245-2· OSTI ID:4686300

Opara-Kubinska, Z; Kurylo-Borowska, Z; Szybalski, W

The transforming activity of 5-bromodeoxyuridinelabeled DNA is more sensitive to ultraviolet light than that of normal (non-labeled) DNA. The more rapid ultravioletirradiation inactivation of 5-bromodeoxyuridine labeled DNA, as compared with nonlabeled, is reflected also by greatly increased affinity of ultraviolet-irradiated 5bromodeoxyuridine-labeled DNA for the methylated albumin column, decrease in density (dehalogenation) and very pronounced band spreading (degradation, increased heterogeneity), as revealed by CsCl equilibrium-density centrifugation of ultraviolet-irradrated native 5-bromodeoxyuridine-labeled DNA's, and marked cross-linking between complementary strands of 6- bromodeoxyuridine-labeled DNA, as determined with ultraviolet-irradiated and denatured material centrifuged in the Cs/sub 2/SO/sub 4/ density gradient. (auth)

• Research Organization: Univ. of Wisconsin, Madison
• NSA Number: NSA-17-028719
• OSTI ID: 4686300
• Journal Information: Biochimica et Biophysica Acta (Netherlands), Journal Name: Biochimica et Biophysica Acta (Netherlands) Vol. Vol: 72; ISSN BBACA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ALBUMINS
BIOCHEMISTRY
BIOLOGY AND MEDICINE
BROMINE COMPLEXES
CENTRIFUGATION
CESIUM CHLORIDES
CESIUM SULFATES
DECOMPOSITION
DNA
EXTRACTION COLUMNS
HETEROCYCLICS
METABOLISM
METHYL RADICALS
NUCLEIC ACIDS
ORGANIC NITROGEN COMPOUNDS
RADIATION EFFECTS
RADIOSENSITIVITY
ULTRAVIOLET RADIATION
URIDINE