ULTRAVIOLET IRRADIATION OF NATIVE AND DENATURED TRANSFORMING DEOXYRIBONUCLEIC ACID FROM HAEMOPHILUS INFLUENZAE
Journal Article
·
· Journal of Bacteriology (U.S.)
OSTI ID:4738225
Two antibiotic-resistance markers in denatured deoxyribonucleic acid (DNA) from Haemophilus influenzae showed about the same sensitivity to ultraviolet irradiation as in native DNA. The resistance of markers in denatured DNA to ultraviolet (UV) light increases with alkalinity up to pH 12. New transforming markers or genetic lethals were not produced by in vitro irradiation of either native or denatured DNA. The UV irradiation of the denatured DNA reduces its capacity to form new markers with nitrous acid. Nitrous acid reactivated some of the intrinsic marker destroyed by UV irradiation of denatured transforming DNA. (auth)
- Research Organization:
- Johns Hopkins Univ., Baltimore
- NSA Number:
- NSA-17-017847
- OSTI ID:
- 4738225
- Journal Information:
- Journal of Bacteriology (U.S.), Journal Name: Journal of Bacteriology (U.S.) Vol. Vol: 85; ISSN JOBAA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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