Transformation of Haemophilus influenzae by recombinant molecules
Conference
·
OSTI ID:6061787
A gene library of Haemophilus influenzae DNA has been constructed by ligating chromosomal DNA from cells resistant to a number of antibiotics, together with DNA of the H. influenzae plasmid RSF0885. Before ligation both DNAs were cut with the enzyme PvuII. The ligated DNA was allowed to enter competent H. influenzae sensitive to the antibiotics and selection was made for resistance to ampicillin, conferred by the plasmid RSF0885 DNA. Plasmids conferring resistance to various other antibiotics, as well as to ampicillin, have been obtained by this procedure and subsequent selection for chromosomal markers.
- Research Organization:
- Brookhaven National Lab., Upton, NY (USA)
- DOE Contract Number:
- AC02-76CH00016
- OSTI ID:
- 6061787
- Report Number(s):
- BNL-29733; CONF-8106127-1; ON: DE81032039
- Country of Publication:
- United States
- Language:
- English
Similar Records
Transformation of Haemophilus influenzae by plasmid RSF0885
Plasmid cloning vehicle for Haemophilus influenzae and Escherichia coli
Physical size of the donor locus and transmission of Haemophilus influenzae ampicillin resistance genes by deoxyribonucleic acid-mediated transformation
Journal Article
·
Mon Nov 30 23:00:00 EST 1981
· J. Bacteriol.; (United States)
·
OSTI ID:5177089
Plasmid cloning vehicle for Haemophilus influenzae and Escherichia coli
Journal Article
·
Wed Sep 01 00:00:00 EDT 1982
· J. Bacteriol.; (United States)
·
OSTI ID:6541598
Physical size of the donor locus and transmission of Haemophilus influenzae ampicillin resistance genes by deoxyribonucleic acid-mediated transformation
Journal Article
·
Wed Dec 31 23:00:00 EST 1975
· J. Bacteriol., v. 125, no. 1, pp. 197-204
·
OSTI ID:4125064