Plasmid cloning vehicle for Haemophilus influenzae and Escherichia coli
Journal Article
·
· J. Bacteriol.; (United States)
OSTI ID:6541598
A new plasmid cloning vehicle (pDM2) was used to introduce a library of Haemophilus influenzae chromosomal fragments into H. influenzae. Transformants of the higly recombination-defective rec-1 mutant were more likely to contain exclusively recombinant plasmids after exposure to ligated DNA mixtures than was the wild type. pDM2 could replicate in Escherichia coli K-12.
- Research Organization:
- Brookhaven National Lab., Upton, NY
- OSTI ID:
- 6541598
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 151:3; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
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