A plasmid carrying mucA and mucB genes from pKM101 in Haemophilus influenzae and Escherichia coli

Journal Article · Sun Oct 01 04:00:00 EDT 1989 · Journal of Bacteriology; (USA)

OSTI ID:5258815

Spikes, D; Setlow, J K ^[1]

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1. Brookhaven National Lab., Upton, NY (USA)

The plasmid pMucAMucB, constructed from the Haemophilus influenzae vector pDM2, and a similar plasmid, constructed from pBR322, increased the survival after UV irradiation of Escherichia coli AB1157 with the umu-36 mutation and also caused UV-induced mutation in the E. coli strain. In H. influenzae, pMucAMucB caused a small but reproducible increase in survival after UV irradiation in wild-type cells and in a rec-1 mutant, but there was no increase in spontaneous mutation in the wild type or in the rec-1 mutant and no UV-induced mutation.

• OSTI ID: 5258815
• Journal Information: Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 171:10; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GENE MUTATIONS
GENES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HAEMOPHILUS
MICROORGANISMS
MUTATIONS
PLASMIDS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
SPONTANEOUS MUTATIONS
SURVIVAL TIME
ULTRAVIOLET RADIATION