Characterization of the rec-1 gene of Haemophilus influenzae and behavior of the gene in Escherichia coli

Journal Article · Thu Sep 01 00:00:00 EDT 1988 · J. Bacteriol.; (United States)

OSTI ID:6745089

Setlow, J K; Spikes, D; Griffin, K

The rec-1 gene of Haemophilus influenzae was cloned into a shuttle vector that replicates in Escherichia coli as well as in H. influenzae. The plasmid, called pRec1, complemented the defects of a rec-1 mutant in repair of UV damage, transformation, and ability of prophage to be induced by UV radiation. Although UV resistance and recombination were caused by pRec1 in E. coli recA mutants, UV induction of lambda and UV mutagenesis were not. We suggest that the ability of the H. influenzae Rec-1 protein to cause cleavage of repressors but not the recombinase function differs from that of the E. coli RecA protein.

• Research Organization: Brookhaven National Lab., Upton, NY (USA)
• OSTI ID: 6745089
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 170:9; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
CLONING
DNA REPAIR
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GENE RECOMBINATION
GENE RECOMBINATION PROTEINS
GENES
GENETIC EFFECTS
GENETIC ENGINEERING
GENETIC RADIATION EFFECTS
HAEMOPHILUS
MICROORGANISMS
MUTAGENESIS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PLASMIDS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
ULTRAVIOLET RADIATION