Transformation of Haemophilus influenzae by plasmid RSF0885

Journal Article · Tue Dec 01 04:00:00 EST 1981 · J. Bacteriol.; (United States)

OSTI ID:5177089

Notani, N K; Setlow, J K; McCarthy, D; Clayton, N L

Plasmid RSF0885, which conferred ampicillin resistance, transformed competent Haemophilus influenzae cells with low efficiency (maximun, less than 0.01%). As judged by competition experiments and uptake of radioactivity, plasmid RSF0885 deoxyribonucleic acid was taken up into competent H. influenzae cells several orders of magnitude less efficiently than H. influenzae chromosomal deoxyribonucleic acid. Plasmid RSF0885 transformed cells with even lower efficiency than could be accounted for by the low uptake. Transformation was not affected by rec-1 and rec-2 mutations in the recipient, and strains cured of the plasmid did not show increased transformation. Plasmid molecules cut once with a restriction enzyme that made blunt ends did not transform. Transformation was favored by the closed circular form of the plasmid.

• Research Organization: Brookhaven National Lab., Upton, NY
• OSTI ID: 5177089
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 148:3; ISSN JOBAA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
CHROMOSOMAL ABERRATIONS
DNA
ENZYME ACTIVITY
HAEMOPHILUS
HYBRIDIZATION
KINETICS
MICROORGANISMS
MUTANTS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLASMIDS
REACTION KINETICS
TEMPERATURE EFFECTS