Restriction enzymes do not play a significant role in Haemophilus homospecific or heterospecific transformation

Journal Article · Fri Oct 01 04:00:00 EDT 1976 · J. Bacteriol.; (United States)

OSTI ID:7349183

Stuy, J H

Competent Haemophilus influenzae Rd recipients, either as phage HP1 restricting (r/sup +/) or nonrestricting (r/sup -/) nonlysogens or defective lysogens, were exposed to deoxyribonucleic acids from various wild-type phage HP1 lysogenic H. influenzae serotype strains (non-encapsulated derivatives of serotypes a, b, c, d, and e), to DNA from lysogenic Haemophilus parahaemolyticus, and to DNA from modified and nonmodified phage HP1. Transformation of antibiotic resistance markers and of prophage markers in homospecific crosses were observed to be unaffected by the recipient restriction phenotype, whereas the transfection response was much reduced in r/sup +/ recipients. Heterospecific transformation of prophage markers was reduced by only 80 to 90 percent, whereas antibiotic resistance marker tranformation was 1,000 to 10,000 times lower. Heterospecific transfection was at least 100 times lower than the homospecific transfection in both r/sup +/ and r/sup -/ recipients. The general conclusion is that neither class I nor class II restriction enzymes affect significantly the transformation efficiency in homospecific and heterospecific crosses. The efficiency of heterospecific transformation may depend mainly on the deoxyribonucleic acid homology in the genetic marker region.

• Research Organization: Florida State Univ., Tallahassee
• OSTI ID: 7349183
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 128:1; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANTIBIOTICS
BACTERIA
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGY
DNA
DRUGS
ENZYMES
GENETICS
HAEMOPHILUS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHENOTYPE
VIRUSES