A COMPARATIVE STUDY OF HUMAN AND MONKEY LEUKOCYTES IN CULTURE
Technical Report
·
OSTI ID:4768649
To develop a satisfactory culture procedure for rhesus monkey (Macaca mulatta) leukocytes, several technical variations of the routine method were investigated. Those included in the present study were the use and the amount of different kinds of phytohemagglutinins, the separation of erythrocytes after preservation or centrifugation, sera derived from the horse, the calf, and the monkey for supplementing the culture medium, and the ratio of host serum containing leukocytes to the nutrient fluid. None of these factors gave satisfactory resuits for monkey samples, but all were useful for human cells. Such an unusual situation might possibly be caused by differences in the physicochemical and cellular properties of the blood of the monkey and of man. (auth)
- Research Organization:
- Pasadena Foundation for Medical Research, Calif.
- NSA Number:
- NSA-17-005676
- OSTI ID:
- 4768649
- Report Number(s):
- SAM-TDR-62-99
- Country of Publication:
- United States
- Language:
- English
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