FURTHER CHROMOSOMAL STUDIES ON IRRADIATED HUMAN LEUKOCYTES IN VITRO

Journal Article · Mon Apr 01 04:00:00 EST 1963 · Idengaku Zasshi (Japan)

DOI:https://doi.org/10.1266/jjg.38.106· OSTI ID:4078315

Awa, A; Ohnuki, Y; Pomerat, C M

Human leukocyte cultures received x irradiation at a dose of 400 r on the 4th day and were fixed on the 6th day. There was an increase from 0.25 to 3.01% in the number of polyploid cells. These were predominantly tetraploids. Microscopic examination revealed that 64% of metaphases observed showed damage. The abnormalities were due to chromosomal breakages, particularly fragments and dicentrics. Counts and detailed analysis of chromosomes in human cells used as controls demonstrated the predominant distribution of euploid elements with 46 chromosomes. These values closely parallel those reported by Ohnuki et al. (1961). In order to develop a satisfactory culture procedure for rhesus monkey (Macaca mulatta) leukocytes, several technical variations of the routine method were investigated. None of these variations gave satisfactory results for monkey samples, but all were useful for human cells. Such an unusual situation might possibly be induced by differences in the physicochemical and cellular properties of monkey blood and that of man. (auth)

• Research Organization: Hokkaido Univ., Sapporo and Pasadena Foundation for Medical Research, Calif.
• Sponsoring Organization: USDOE
• NSA Number: NSA-18-015840
• OSTI ID: 4078315
• Journal Information: Idengaku Zasshi (Japan), Journal Name: Idengaku Zasshi (Japan) Vol. Vol: 38; ISSN IDZAA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BIOCHEMISTRY
BIOLOGY AND MEDICINE
BLOOD
CHROMOSOMES
LEUCOCYTES
MACACA MULATTA
MAN
MICROSCOPY
MITOSIS
MONKEYS
RADIATION DOSES
RADIATION EFFECTS
VARIATIONS
X RADIATION