Chromosomal studies on irradiated leukocytes in vitro

Journal Article · Wed Nov 01 04:00:00 EST 1961 · Annals of the New York Academy of Sciences

DOI:https://doi.org/10.1111/j.1749-6632.1961.tb50084.x· OSTI ID:4805850

Ohnuki, Y.; Awa, A.; Pomerat, C. M.

The technique for the cultivation of human leukocytes with the use of heparinized venous blood treated with phytohemagglutinin is described. With this method chromosome spreads were made from 6-day cultures using the colchicine- hypotonic sodium citrate-acetic orcein-squash sequence. The range of polyploidy was determined on 2 specimens for each of 3 individuals. Detailed analyses and counts of human chromosomes indicated the predominant distribution to be euploid cells having 46 chromosomes. Idiogram studies showed the same basic pattern demonstrated by previous investigators. These data served as a basis for comparing results obtained from sister cultures irradiated with 400 r from a cobalt-60 source on the fourth day and fixed on the sixth day. Under these experimental conditions there was a considerable increase, 6.3%, in the number of polyploid cells. Among these, tetraploid cells were the most frequent, being 5.4%. By chromosomal analyses, 56.6% of the cells were found to be damaged. Most injuries were due to chromosomal breakages, especially fragments and dicentrics. Endoreduplication also was noted frequently.

• Research Organization: Pasadena Foundation for Medical Research, Calif.
• Sponsoring Organization: USDOE
• NSA Number: NSA-16-026759
• OSTI ID: 4805850
• Journal Information: Annals of the New York Academy of Sciences, Journal Name: Annals of the New York Academy of Sciences Journal Issue: 2 Vol. 95; ISSN 0077-8923
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
CHROMOSOMES
COBALT 60
GAMMA RADIATION
LEUCOCYTES
MAN
QUANTITY RATIO
RADIATION EFFECTS
RADIATION INJURIES