IRRADIATION-INDUCED CHROMOSOME ABERRATIONS IN NORMAL HUMAN LEUKOCYTES IN CULTURE

Journal Article · Sat Sep 01 04:00:00 EDT 1962 · Canadian Journal of Genetics and Cytology (Canada)

DOI:https://doi.org/10.1139/g62-044· OSTI ID:4743871

Bell, A G; Baker, D G

An investigation of irradiation-induced chromosome aberrations in human leukocytes in short-term culture is described. In the production of one-hit aberrations there appeared to be a linear response to dose, but no dose-rate effect. The data were compatible with either a linear or a possible power- function relationship between dose and aberration frequency in the production of two-hit aberrations. The small sample size was considered to be the most probable explanation for this lack of discrimination. A defintte dose-rate response was observed in the production of twohit aberrations, the larger number being produced at the higher dose-rate. It is difficult to compare induction rates as derived from different laboratories, owing possibly to differert methods of scoring, and also to attempt to derive break-rates'' by combining one-hit and two-hit aberration data. Because of a definite dose-rate effect on the production of two-hit aberrations, and also because these are expected to occur as a power-function of the dose, induction rates might more properly be calculated for each type of aberration separately. These studies indicate that irradiation-induced polyploidy follows from the initial induction of endoreduplication. The chromosome pairs of the endoreduplicated cells appear to fall apart at the first mitotic division following diplochromosome induction, to result in tetraploid daughter cells. Short-term cultures of human leukocytes provide a satisfactory system in which to examine irradiation effects. (auth)

• Research Organization: Research Inst. of the Hospital for Sick Children, Toronto; Banting and Best Dept. of Medical Research, Toronto
• Sponsoring Organization: USDOE
• NSA Number: NSA-17-007826
• OSTI ID: 4743871
• Journal Information: Canadian Journal of Genetics and Cytology (Canada), Journal Name: Canadian Journal of Genetics and Cytology (Canada) Vol. Vol: 4; ISSN CNJGA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BIOLOGY AND MEDICINE
CHROMOSOMES
LEUCOCYTES
MAN
MITOSIS
RADIATION DOSES
RADIATION EFFECTS
RADIATION INJURIES