STUDIES ON THE METABOLISM OF RADIOACTIVE STRONTIUM Sr$sup 89$$,$$sup 90$. II. STUDIES ON THE BINDING POTENCY OF Sr$sup 89$ TO BONE TISSUE IN VITRO
The dye bath method was used to study the binding of Sr/sup 89/ to guinea pig bone slices (fresh bone and dead bone heated in distilled water at 100 deg C for 1 hr) to elucidate the mechanism of Sr/sup 89/ deposition in the skeleton and the action of various chemical agents that remove Sr. When the slices were incubated at 37.0 deg in physiological saline solution or 1/5 M tris buffer solution (pH 7.24) containing Sr/sup 89/Cl/sub 2/, the binding rate of Sr with the slices could be expressed as an exponential function for 4 hr from the beginning of incubation. The binding rate of Sr and bone slices at 37.0 deg was higher than that at 27.4 deg , that is, the reaction is endothermic. Sr/sup 89/ was first bound in the organic fraction and then migrated to the mineral fraction of bone tissue. EDTA and BAL prevented Sr binding to bone by forming chelate complexes with Sr. Zirconium citrate and calcium chloride acted by competition or an ion-exchange reaction with Sr in the organic fraction of bone tissue. Sodium citrate accelerated the excretion of Sr in vivo, but was not effective in preyenting the binding of Sr to bone slices in vitro. The mechanism of these phenomena is not yet clear, but it may be concluded that the affinity of bone mineral for sodium citrate is greater than that for Sr. Parathyroid hormone had no effect on the binding of Sr/sup 89/ by bone slices in vitro. (H.H.D.)
- Research Organization:
- Kyoto Univ.
- NSA Number:
- NSA-17-012108
- OSTI ID:
- 4763581
- Journal Information:
- Acta Schol. Med. Univ. Kioto, Journal Name: Acta Schol. Med. Univ. Kioto Vol. Vol: 37
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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