PARADOXICAL POTENTIATION OF RADIOINACTIVATION OF SULFHYDRYL ENZYMES BY CATALASE (in German)
Catalase was previously shown to inhibit the radiationinduced suppression of glycolysis in ascites tumor cells and the suppression of methemoglobin formation in irradiated erythrocytes. However, in experiments with liver alcohol dehydrogenase, catalase showed in opposite effect, i.e., it promoted radioinactivation. Addition of 10 mu g liver catalise per ml to a solution of 80 mu g/ml liver alcohol dehydrogenase reduced the activity of the latter enzyme to 22% of normal after irradiation with 5000 r, compared with 36% in the absence of catalase. Addition of heat-inactivated catalase or serum albumin did not inhibit the radiationinduced reduction in activity. Similar results were obtained with another sulfhydryl-dependent enzyme, lactic dehydrogenase. Whereas catalase may promote the destruction of H/sub 2/O/sub 2/ formed during irradiation from OH radicals, it may also promote the oxidation enzyme sulfhydryl groups by the irradiation products. (H.H.D.)
- Research Organization:
- Medizinisch-chemisches Institut der Universitat, Bern
- NSA Number:
- NSA-17-002847
- OSTI ID:
- 4757946
- Journal Information:
- Helvetica Physiologica et Pharmacologica Acta (Switzerland) Ceased publication, Journal Name: Helvetica Physiologica et Pharmacologica Acta (Switzerland) Ceased publication Vol. Vol: 19; ISSN HPPAA
- Country of Publication:
- Country unknown/Code not available
- Language:
- German
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