EFFECT OF Co$sup 60$ GAMMA RADIATION ON CATALASE (in Rumanian)
Abstract
Catalase, isolated in crystalline form from equine, porcine, and avian liver and from equine erythrocytes was dissolved in 0.1M phosphate buffer at pH 7.4 and exposed to CO/sup 60/ gamma radiation at a dose rate of 5 mr/hr. Changes in the activity of the enzyme were studied polarographically after 30-min (2.5 mr) irradiation. Polarograms were obtained with a solution of hydrogen peroxide at 8.8 x 10/sup -4/M, and with the same solution after addition of native or irradiated peroxidase. The amplitude of the polarographic halfwave, particularly at lower voltages, was greater for the irradiated than for the native enzyme for liver, whereas it smaller in the case of the erythrocyte enzyme. These results indicate that the liver enzyme of three species was inactivated by gamma radiation, while red cell catalase was activated by the same treatment. Similar evidence of activation of red cell catalase was obtained when it was shaken with a 1: 1 mixture of chloroform and ethanol. The divergent behavior of liver and erythrocyte catalases toward irradiation is discussed in terms of the differences in the electronic structure of their prosthetic heme groups. (BBB)
- Authors:
- Publication Date:
- Research Org.:
- Facultatea de Medicina Veterinara, Bucharest
- OSTI Identifier:
- 4647687
- NSA Number:
- NSA-17-037071
- Resource Type:
- Journal Article
- Journal Name:
- Acad. Rep. Populare Romine, Studii Cercetari Biochim.
- Additional Journal Information:
- Journal Volume: Vol: 5; Other Information: Orig. Receipt Date: 31-DEC-63
- Country of Publication:
- Country unknown/Code not available
- Language:
- Rumanian
- Subject:
- BIOLOGY AND MEDICINE; BIOCHEMISTRY; CATALASE; COBALT 60; ENZYMES; ERYTHROCYTES; GAMMA RADIATION; LIVER; POLAROGRAPHY; PREPARATION; RADIATION DOSES; RADIATION EFFECTS; SOLUTIONS; VARIATIONS
Citation Formats
Buruiana, L M, and Pavlu, V. EFFECT OF Co$sup 60$ GAMMA RADIATION ON CATALASE. Country unknown/Code not available: N. p., 1962.
Web.
Buruiana, L M, & Pavlu, V. EFFECT OF Co$sup 60$ GAMMA RADIATION ON CATALASE. Country unknown/Code not available.
Buruiana, L M, and Pavlu, V. 1962.
"EFFECT OF Co$sup 60$ GAMMA RADIATION ON CATALASE". Country unknown/Code not available.
@article{osti_4647687,
title = {EFFECT OF Co$sup 60$ GAMMA RADIATION ON CATALASE},
author = {Buruiana, L M and Pavlu, V},
abstractNote = {Catalase, isolated in crystalline form from equine, porcine, and avian liver and from equine erythrocytes was dissolved in 0.1M phosphate buffer at pH 7.4 and exposed to CO/sup 60/ gamma radiation at a dose rate of 5 mr/hr. Changes in the activity of the enzyme were studied polarographically after 30-min (2.5 mr) irradiation. Polarograms were obtained with a solution of hydrogen peroxide at 8.8 x 10/sup -4/M, and with the same solution after addition of native or irradiated peroxidase. The amplitude of the polarographic halfwave, particularly at lower voltages, was greater for the irradiated than for the native enzyme for liver, whereas it smaller in the case of the erythrocyte enzyme. These results indicate that the liver enzyme of three species was inactivated by gamma radiation, while red cell catalase was activated by the same treatment. Similar evidence of activation of red cell catalase was obtained when it was shaken with a 1: 1 mixture of chloroform and ethanol. The divergent behavior of liver and erythrocyte catalases toward irradiation is discussed in terms of the differences in the electronic structure of their prosthetic heme groups. (BBB)},
doi = {},
url = {https://www.osti.gov/biblio/4647687},
journal = {Acad. Rep. Populare Romine, Studii Cercetari Biochim.},
number = ,
volume = Vol: 5,
place = {Country unknown/Code not available},
year = {Mon Jan 01 00:00:00 EST 1962},
month = {Mon Jan 01 00:00:00 EST 1962}
}