STUDIES ON THE CATALASE ACTIVITY OF CHEMOANTIBIOTIC-RESISTANT MYCOBACTERIA SUBJECTED TO X IRRADIATION

Journal Article · Fri Mar 15 04:00:00 EST 1963 · Boll. Soc. Ital. Biol. Sper.

OSTI ID:4024506

Paolini, F A; Moncalvo, F; Moreo, G C

An earlier study on a strain of Mycobacterium tuberculosis (ATCC-607) resistant to streptomycin (1000 mu g/ml) showed that x irradiation with a dose of 60,000 r caused a total loss of catalase activity. Further studies were conducted on five human strains of mycobacteria which were resistant respectively to 100 mu g/ml kanamycin, 10 mu g/ml isonlazid, 250 mu g/ml streptomycin, 250 mu g/ral p-aminosalicylate, and 10 mu g/ml streptomycin + 25 mu g/ml kanamycin. Resistance in these strains was developed in vitro by addition of increasing amounts of these compounds. The cultures were exposed to x-ray doses of 1000 to 80,000 r, and catalase activity was determined by the manganometric method. Irradiation reduced the enzyme activity and doses of 50,000 r in some strains and 60,000 r in others completely abolished activity. The kanamycin- and isoniazidresistant strains showed greater loss of catalase activity than the other strains. No relation was found between the degree of resistance and enzyme activity or its response to irradiation. (BBB)

• Research Organization: Universita, Pavia, Italy
• NSA Number: NSA-18-019607
• OSTI ID: 4024506
• Journal Information: Boll. Soc. Ital. Biol. Sper., Journal Name: Boll. Soc. Ital. Biol. Sper. Vol. Vol: 39
• Country of Publication: Country unknown/Code not available
• Language: Italian

Related Subjects

AMINES
ANTIBIOTICS
BACTERIA
BIOCHEMISTRY
BIOLOGY
BIOLOGY AND MEDICINE
CATALASE
CHEMICAL REACTIONS
DRUGS
ENZYMES
IRRADIATION
ISONIAZID
MEASURED VALUES
MEDICINE
MYCOBACTERIUM TUBERCULOSIS
PAS
PERFORMANCE
RADIATION DOSES
RADIOSENSITIVITY
SALICYLIC ACID
SENSITIVITY
STREPTOMYCIN
VARIATIONS
X RADIATION