Mercuric reductase activity and evidence of broad-spectrum mercury resistance among clinical isolates of rapidly growing mycobacteria
Journal Article
·
· Antimicrobial Agents and Chemotherapy; (United States)
- Univ. of Texas Health Center, Tyler (USA)
Resistance to mercury was evaluated in 356 rapidly growing mycobacteria belonging to eight taxonomic groups. Resistance to inorganic Hg2+ ranged from 0% among the unnamed third biovariant complex of Mycobacterium fortuitum to 83% among M. chelonae-like organisms. With cell extracts and 203Hg(NO3)2 as the substrate, mercuric reductase (HgRe) activity was demonstrable in six of eight taxonomic groups. HgRe activity was inducible and required NADPH or NADH and a thiol donor for optimai activity. Species with HgRe activity were also resistant to organomercurial compounds, including phenylmercuric acetate. Attempts at intraspecies and intragenus transfer of HgRe activity by conjugation or transformation were unsuccessful. Mercury resistance is common in rapidly growing mycobacteria and appears to function via the same inducible enzyme systems already defined in other bacterial species. This system offers potential as a strain marker for epidemiologic investigations and for studying genetic systems in rapidly growing mycobacteria.
- OSTI ID:
- 5263678
- Journal Information:
- Antimicrobial Agents and Chemotherapy; (United States), Journal Name: Antimicrobial Agents and Chemotherapy; (United States) Vol. 35:5; ISSN AMACC; ISSN 0066-4804
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MARKERS
DAYS LIVING RADIOISOTOPES
ELEMENTS
ENERGY
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
HEAT
HEAVY NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MERCURY
MERCURY 203
MERCURY ISOTOPES
METALS
MICROORGANISMS
MYCOBACTERIUM
NUCLEI
OXIDOREDUCTASES
RADIOISOTOPES
TOLERANCE
TOXICITY
TRACER TECHNIQUES
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MARKERS
DAYS LIVING RADIOISOTOPES
ELEMENTS
ENERGY
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
HEAT
HEAVY NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MERCURY
MERCURY 203
MERCURY ISOTOPES
METALS
MICROORGANISMS
MYCOBACTERIUM
NUCLEI
OXIDOREDUCTASES
RADIOISOTOPES
TOLERANCE
TOXICITY
TRACER TECHNIQUES