EFFECTS OF X-RADIATION AND OF METABOLIC INHIBITORS ON RAT THYMOCYTES IN VITRO

Journal Article · Thu Nov 01 04:00:00 EST 1962 · Can. J. Biolchem. Physiol.

DOI:https://doi.org/10.1139/o62-174· OSTI ID:4755151

Myers, D K; DeWolfe, D E; Araki, K

The survival of x irradiated thymocytes in vitro was studied after incubation for various periods of time and at different pH values as well as at different temperatures. Death at 25 deg C following exposure to high doses of x radiation may be due to the liberation of destructive enzymes. The effects of high doses could be largely prevented by addition of nicotinamide after irradiation. The cause of cell death at 37 deg C following exposure to low doses of x radiation is still uncertain. Some metabolic inhibitors also showed a considerable difference in their toxicity to thymocytes at 37 deg C and at 25 deg C. Dinitrophenol, for example, was highly toxic at 37 deg C but almost ineffective in killing cells at 25 deg C, even though it produced similar decreases in ATP levels at both temperatures. The marked difference in survival of irradiated thymocytes at 37 deg C and at 25 deg C could therefore be explained if low doses of x radiation interfered with the normal cell metabolism. However, low doses of radiation, unlike dinitrophenol, did not produce any immediate decrease either in ATP levels or in oxygen consumption of thymocyte suspensions. (auth)

• Research Organization: Atomic Energy of Canada Ltd., Chalk River, Ont.
• Sponsoring Organization: USDOE
• NSA Number: NSA-17-007823
• OSTI ID: 4755151
• Journal Information: Can. J. Biolchem. Physiol., Journal Name: Can. J. Biolchem. Physiol. Vol. Vol: 40
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ACIDITY
ANIMAL CELLS
BIOLOGY AND MEDICINE
CHEMICALS
DNP
ENZYMES
METABOLISM
ORGANIC NITROGEN COMPOUNDS
OXIDATION
PHENOLS
RADIATION EFFECTS
RADIATION PROTECTION
TEMPERATURE
TOXICITY
VITAMINS
X RADIATION