Aerobic glycolysis of x-irradiated thymocytes

Journal Article · Tue Jan 01 04:00:00 EST 1963 · Biochemistry and Cell Biology

DOI:https://doi.org/10.1139/o63-242· OSTI ID:4623402

Araki, Kimiko; Myers, D. K.

The aerobic lactate production of rat thymocyte suspensions incubated at 37 deg C for 2 hours was doubled following exposure to approximately 70 r x radiation. Lower doses down to 18 r also produced a significant increase in aerobic lactate production. Increased lactate accumulation following exposure to 1000 r was observed after incubation for as little as 30-60 minutes, though the rate of accumulation increased still further between 2 and 4 hours incubation. A decrease in pH or temperature during incubation of irradiated thymocyte suspensions minimized the lactic acid accumulation. A comparison of the effects of x irradiation in different media and of the effects of several metabolic inhibitors suggested that the increase in aerobic lactate production was a sensitive indicator of cell damage associated with the loss of intracellular potassium ions. Respiration and anaerobic glycolysis of the thymus cells were both much less sensitive than aerobic glycolysis to the effects of x irradiation. The anaerobic lactate production of rat thymocyte suspensions in the presence of dinitrophenol was reduced by 50% after exposure to 50006000 r of x radiation.

• Research Organization: Atomic Energy of Canada Ltd., Chalk River, Ont.
• Sponsoring Organization: USDOE
• NSA Number: NSA-17-040577
• OSTI ID: 4623402
• Journal Information: Biochemistry and Cell Biology, Journal Name: Biochemistry and Cell Biology Journal Issue: 10 Vol. 41; ISSN 0829-8211
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ACIDITY
ANIMAL CELLS
GLYCOLYSIS
IONS
IRRADIATION
LACTIC ACID
METABOLISM
ORGANIC ACIDS
ORGANIC NITROGEN COMPOUNDS
OXIDATION
OXYGEN
PHENOLS
POTASSIUM
RADIATION DOSES
RADIATION EFFECTS
RADIATION INJURIES
RADIOSENSITIVITY
RATS
SUGARS
SUSPENSIONS
TEMPERATURE
THYMUS
TISSUES
X RADIATION