Changes in ATP metabolism in x-irradiated rat thymocytes (in Japanese)
When animals are irradiated, the initial radiation damage to molecules, in any type of cell is presumably similar. Yet, the marner in which cells die varies. Cell death can be divided into two classes, interphase death and reproductive death. The importance of interphase death in radiobiology is seen in its role in the radioinduced death of animals. There are probably three types of interphase death. To study the first type of interphase death, thymocytes and lymphocytes in vivo and in vitro are used. Lesions in ATP metabolism have been implicated in the mechanism leading to interphase death that occurs in thymocytes shortly after x irradiation. The author and her co-workers found the existence of lactate and fructose-1,6-diphosphate accumulation caused by enhanced glycolysis in the x-irradiated rat thymocytes and also found that ATP level was considerably decreased after irradiation, whereas ADP and AMP levels remained practically unchanged. This means that the degradation of the nucleotides proceeded beyond the level of nucleosides or purinebases. Hypoxanthine began to be released into the extracellular medium in the early phase of 2 hrs' incubation at 37 deg C with glucose after irradiation. The amount was dependent on the x- ray doses ranging from 0.5 kR to 32 kR and may possibly explain the loss of the adenine nucleotides after irradiation. Addition of 5 mM adenine to 1 kR- irradiated-rat thymocytes restored the ATP-content to normal level. Carbon-14- labeled adenine was incorporated into ATP and the ATP thus labeled degraded to hypoxanthine. Adenine in 5 mM concentration did not enhance the ATP synthesis from /sup 14/C-adenine, but depressed markedly the degradation of ATP to hypoxanthine. It was concluded that restoration of the ATP level by adenine resulted from its depressive effect on degradation of adenine nucleotides. (auth)
- Research Organization:
- Tokyo Women's Medical Coll.
- NSA Number:
- NSA-29-010444
- OSTI ID:
- 4362640
- Journal Information:
- Tokyo Joshi Ika Daigaku Zasshi, v. 42, no. 10, pp. 725-737, Journal Name: Tokyo Joshi Ika Daigaku Zasshi, v. 42, no. 10, pp. 725-737; ISSN TJIZA
- Country of Publication:
- Japan
- Language:
- Japanese
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*THYMOCYTES-- RADIATION INJURIES
ADENINES
BIOSYNTHESIS
CELL KILLING
CELL MEMBRANES
CELL NUCLEI
DNA
GLYCOLYSIS
LACTIC ACID
MITOSIS
N48520* --Life Sciences--Radiation Effects on Animals-- Vertebrates
PHOTON BEAMS
RATS
SUBLETHAL IRRADIATION
TEMPERATURE DEPENDENCE
TIME DEPENDENCE
X RADIATION