Reduction of rat thymocyte interphase death by hyperthermia

Journal Article · Thu May 01 04:00:00 EDT 1980 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575384· OSTI ID:5162958

Ohyama, H; Yamada, T

The effects of hyperthermia on rat thymocytes subjected to 1 kR irradiation were examined. Radiation-induced interphase death (judged by dye staining and ATP decline) observed after 4 hr of incubation of 37/sup 0/C following irradiation was reduced by exposing the cells for 30 min to 1 hr to 43/sup 0/C immediately before or after irradiation. Hyperthermia treatment at a lower temperature (such as 41/sup 0/C) was also effective in inducing cell recovery from radiation damage, although a longer treatment was needed. Hyperthermia administered 2 h after irradiation was ineffective and even enhanced cell death. To elucidate the mechanism underlying the effect, (/sup 14/C)adenine and (/sup 32/P)orthophosphate were used to estimate changes in ATP metabolism after heat exposure. Heat alone stimulated both ATP synthesis and degradation regardless of irradiation. Radiation-induced ATP degradation was markedly depressed by heat treatment.

• Research Organization: National Inst. of Radiological Sciences, Chiba, Japan
• OSTI ID: 5162958
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 82:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ATP
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOSYNTHESIS
BODY TEMPERATURE
DEATH
HYPERTHERMIA
INJURIES
MAMMALS
NUCLEOTIDES
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATION INJURIES
RADIOSENSITIVITY EFFECTS
RATS
RECOVERY
RODENTS
SOMATIC CELLS
SYNTHESIS
THYMOCYTES
TOXICITY
VERTEBRATES