Prevention of interphase death in rat thymocytes by bisulfite
The effect of sodium bisulfite, a specific inhibitor of chromatin proteolysis, on radiation damage in rat thymocytes in vitro was examined. Rat thymocytes irradiated with 1 kR X rays in vitro were incubated at 37 degrees C with 10 mM glucose for 4 to 6 hr. During that time development of interphase death as judged by erythrosin B uptake, release of low molecular weight DNA (free DNA), and reduction in cell size was measured. Sodium bisulfite added to the cells at the beginning of incubation exerted a marked preventive effect on radiation damage. The effect was enhanced with increasing concentration of bisulfite from 0.25 to 2 mM. The effect of bisulfite was reversible; i.e., removal of bisulfite from the cells resulted in the reappearance of the radiation damage.
- Research Organization:
- Division of Radiation Health, National Institute of Radiological Sciences, Anagawa, Chiba, Japan
- OSTI ID:
- 5605085
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 95:1; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHROMATIN
DECOMPOSITION
DNA
DYES
ELECTROMAGNETIC RADIATION
ERYTHROSINE
FLUORESCEIN
HYDROXY ACIDS
HYDROXY COMPOUNDS
IONIZING RADIATIONS
MAMMALS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
OXYGEN COMPOUNDS
PHENOLS
POLYPHENOLS
PROTEOLYSIS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RATS
RODENTS
SOMATIC CELLS
SULFITES
SULFUR COMPOUNDS
THYMOCYTES
UPTAKE
VERTEBRATES
X RADIATION