Prevention of interphase death in rat thymocytes by bisulfite

Journal Article · Fri Jul 01 04:00:00 EDT 1983 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576076· OSTI ID:5598127

Ohyama, H ^[1]; Shimizu, M; Yamada, T

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1. National Inst. of Radiological Sciences, Anagawa, Japan

The effect of sodium bisulfite, a specific inhibitor of chromatin proteolysis, on radiation damage in rat thymocytes in vitro was examined. Rat thymocytes irradiated with 1 kR x rays in vitro were incubated at 37/sup 0/C with 10 mM glucose for 4 to 6 h. During that time development of interphase death as judged by erythrosin B uptake, release of low molecular weight DNA (free DNA), and reduction in cell size was measured. Sodium bisulfite added to the cells at the beginning of incubation exerted a marked preventive effect on radiation damage. The effect was enhanced with increasing concentration of bisulfite from 0.25 to 2 mM. The effect of bisulfite was reversible; i.e., removal of bisulfite from the cells resulted in the reappearance of the radiation damage.

• OSTI ID: 5598127
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 95:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL COMPOUNDS
ANIMAL CELLS
ANIMALS
CELL KILLING
ELECTROMAGNETIC RADIATION
IN VITRO
IONIZING RADIATIONS
MAMMALS
OXYGEN COMPOUNDS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
RATS
RODENTS
SODIUM COMPOUNDS
SOMATIC CELLS
SULFITES
SULFUR COMPOUNDS
THYMOCYTES
VERTEBRATES
X RADIATION