SELECTIVE IRRADIATION OF LYMPHATIC TISSUES IN PREPARATION FOR TISSUE HOMOGRAFTING
A radiation procedure that delivers a larger dose of radiation to lymphatic structures than to other radiosensitive nontarget tissues such as bone marrow or gastrointestinal tract is described. When Y/sup 90/ is chelated with diethylenetriaminepentaacetic acid (DTPA) and administered intravenously, it is found in greater concentration in lymph nodes than in bone marrow or in the ileum. The relative dosages received by various tissues when 10 mC/lb body wt of Y/sup 90/-DTPA is administered in the dog are: renal cortex 1540 rad, lung 525, gastric mucosa 435, lymph nodes 400, ileum 375, liver 240, bone marrow 115 to 225, muscle 155, and spinal cord 20. The relatively high dose of radiation received by the renal cortex was not found to be prohibitive. It is necessary to reinfuse Y/sup 90/ -DTPA at a rate equal to its rate of excretion in order to maintain a constant level of radioactivity. Four human subjects and 50 dogs were irradiated using the procedure with intravenous urine cycling maintained for 6 hr. The procedure itself was well tolerated in all instances and appeared to be innocuous. Dogs were given doses of radiation in the sublethal and lethal ranges. With an estimated dose of 150 to 210 rad to their lymph nodes all survived over 6 weeks following radiation. In terms of biologic effect, the dosage to lymph nodes is roughly equivalent to more than twice its value of supervoltage x rays. Administration of autologous bone marrow resulted in the survival of the lethally radiated dogs with the return of circulating granulocytes, reticulocytes, and platelets, and absence of significant gastrointestinal symptoms. The circulating lymphocytes, however, failed to show a marked increase. Thus, the critical factor in the death of animals given lethal doses of Y/sup 90/-DTPA is damage to bone marrow rather than to lymphatic tissue or gastrointestinal mucosa. Four patients with acute lymphatic leukemia treated with Y/sup 90/-DTPA survived the urine cycling well and remissions of their leukemia were obtained. It is suggested that the procedure is clinically applicable and offers promise in the preparation of human beings for homografting of tissues. (TCO)
- Research Organization:
- Univ. of California, Berkeley
- NSA Number:
- NSA-17-021480
- OSTI ID:
- 4728096
- Journal Information:
- American Journal of Surgery (U.S.), Journal Name: American Journal of Surgery (U.S.) Vol. Vol: 105; ISSN AJSUA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
RELATIVELY SELECTIVE BETA IRRADIATION OF LYMPHATIC STRUCTURES IN THE DOG USING Y$sup 90$-DTPA
AUTOGRAFTS OF BONE MARROW IN DOGS AFTER LETHAL TOTAL-BODY RADIATION
Related Subjects
AMINES
BIOLOGY AND MEDICINE
BLOOD CELLS
BLOOD FORMATION
BONE MARROW
CHELATES
DOGS
DTPA
ERYTHROCYTES
ETHYLENE
GRANULOCYTES
INTESTINE
KIDNEYS
LETHAL DOSE
LEUCOCYTES
LEUKEMIA
LIVER
LUNGS
LYMPH SYSTEM
LYMPHOCYTES
MAN
MEDICINE
MUSCLES
NERVOUS SYSTEM
PLATELETS
RADIATION DOSES
RADIOSENSITIVITY
RECOVERY
RETICULOCYTES
SPINAL CORD
STOMACH
SURVIVAL TIME
TISSUES
TRANSPLANTS
YTTRIUM 90