AUTOGRAFTS OF BONE MARROW IN DOGS AFTER LETHAL TOTAL-BODY RADIATION

Journal Article · Mon Feb 01 04:00:00 EST 1960 · Blood (U.S.)

OSTI ID:4143096

Mannick, J A; Lochte, Jr, H L; Ashley, C A; Thomas, E D; Ferrebee, J W

Four bergles were exposed to supralethal total-body irradiation from a 250 kw x-ray therapy machine. Four were exposed to similar irradiation from a cobalt-60 teletherapy unit. Dosages administsred ranged from 600 to 1500 r, calculatsd as air dose at theoretical atidbody line. After irradiation each dog was given an intravenous infusion of 1.4 to 3.7 billion cells of his own bone marrow. Tlds sample of autologous marrow was aspirated through a surgical window in the femur immediately prior to the animal's irradiation and was stored in plasma and tissue culture fluid at 4 C while awaiting use. All dogs survived the acuts radiation injury. There were two late deaths, one from intercurrent infection and one from small bowel obstruction. The six surviving dogs are in apparent good health 5 months to 10 months aftsr irradiation. Control dogs in this laboratory have a 100 percent 15-day mortality after air dose of 600 r total- body irradiation. A comparison with a previous series of dogs grafted with homologous marrow shows that autologous marrow induces a recovery after lethal irradiation that is superior to the recovery induced by homologous marrow. The superior results observed after autologous marrow appear attributable to a rapid postirradiation recovery of lymphoid tissue as well as bone marrow. Circulating lymphocytes reappear, lymph nodes regain normal histologic appearance, and the serunn gamma globulin pnttsrn returns to normal. These lattsr events do not occur promptly after infusions of homologous marrow. (auth)

• Research Organization: Mary Imogene Bassett Hospital, Cooperstown, N.Y.
• NSA Number: NSA-14-013595
• OSTI ID: 4143096
• Journal Information: Blood (U.S.), Journal Name: Blood (U.S.) Vol. Vol: 15; ISSN BLOOA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BEAGLES
BIOLOGY AND MEDICINE
BLOOD PLASMA
BODY
BONE MARROW
BONES
COBALT 60
DOGS
FLUIDS
GLOBULINS
INFECTIONS
INTESTINE
IRRADIATION
LETHAL DOSE
LYMPH SYSTEM
LYMPHOCYTES
RADIATION DOSES
RADIATION INJURIES
RECOVERY
STORAGE
SURGERY
TEMPERATURE
TISSUES
TRANSPLANTS
X RADIATION