IN VITRO AND IN VIVO ASSESSMENT OF THE VIABILITY OF DOG MARROW AFTER STORAGE

Journal Article · Fri Apr 01 04:00:00 EST 1960 · Blood (U.S.)

OSTI ID:4143093

Mannick, J A; Lochte, Jr, H L; Thomas, E D; Ferrebee, J W

Assessment was made of the abitity of dog marrow cells to survive sterrge at 4 deg C and at --79 deg C. Survival has been assessed in vitro by measurements of rates of DNA synthesis and in vivo by ability to restore marrow functions in lethally irradiated recipients. Dog bone marrow cells synthesize DNA in vitro at values 30 to 80% of normal after storrge for 24 hours at 4 deg C. DNA synthesis is reduced to very low levels by storage for 96 hours at 4 deg C. Eight lethally irradiated bergle dogs (600 to 1500 r) survived acute irradiation effects and returned to normal health after receiving intravenous infusions of autologous bone marrow that had been stored at 4 deg C for 24 hours or less. One dog, similarly irradiatsd, failed to survive when given marrow stored for 96 hours at 4 deg C. A tenth dog did survive when given an exceptionally large quantity of marrow stored for 96 hours at 4 deg C. Dog marrow cells synthesize DNA in vitro at values 40 to 50% of normal in samples stored at --79 deg C in 15% glycerol for periods up to 17 days. Five lethally irradiatsd beagle dogs (1000 to 1500 r) survived acuts irradiation effects and returned to normal health after receiving intravenous infusions of autologous marrow that had been stored at --79 deg C in 15% glycerol. One other dog, similarly irradiated and treated, regained marrow function but died of hepatitis. Two other irradiated dogs similarly treated did not regain marrow function and died. These two had received exceptionally small infusions of marrow cells. The observations demonstrate that dog marrow can be preserved satisfactorily at 4 deg C for 24 hours and in glycerol at --79 deg C for longer periods. The capacity of the stored marrow to synthesize DNA in vitro has correlated well with its ability to induce recovery in lethally irradiated autologous recipients. DNA synthesis in vitro appears to be a useful index of the ability of marrow cells to proliferate in vivo. (auth)

• Research Organization: Mary Imogene Bassett Hospital, Cooperstown, N.Y.
• NSA Number: NSA-14-013596
• OSTI ID: 4143093
• Journal Information: Blood (U.S.), Journal Name: Blood (U.S.) Vol. Vol: 15; ISSN BLOOA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ALCOHOLS
ANIMAL CELLS
BIOCHEMISTRY
BIOLOGY AND MEDICINE
BONE MARROW
DISEASES
DNA
DOGS
GLYCERIN
IRRADIATION
LETHAL DOSE
LIVER
LOW TEMPERATURE
MEASURED VALUES
NUCLEIC ACIDS
RADIATION EFFECTS
RECOVERY
STORAGE
TEMPERATURE
TRANSPLANTS