VALUE OF BONE MARROW AND SPLEEN CELL SUSPENSIONS FOR SURVIVAL OF LETHALLY IRRADIATED DOGS
The protective effect of homologous spleen and homologous marrow and of autologous spleen and autologous marrow was studied in 85 mongrel dogs after their single exposure to lethal doses of total-body x radiation. In the autologous experiments the material was administered intravenously and intra- arterially immediately after irradiation, while in the homologous experiments the cell suspensions were given as late as 24 hr after irradiation. No protection was noted with these techniques at radiation dose levels of 600, 550, and 450 r. Survival was somewhat longer at 450 r than at 600 r for both treated and control groups. Within the ciable difference in survival at these radiation dosage levels. The delayed administration of homologous marrow up to 24 hours after irradiation and the use of antibiotics and cortisone were likewise without significant protection. In most animals of all groups, there was depression of the white blood cell count to less than 1,000 cells per mm by the 6th to 9th day, and at post-mortem examination, marrow and lymphoid aplasia were found. Significant protection was noted when autologous marrow was used at radiation dose levels of 400 r. White cell oount depression was less severe for the treated than for the control group. Surviving dogs were killed and were found to have normal hematopoietic and lymphatic systems. It is concluded that viable homologous or autologous spleen and marrow cell suspensions failed to protect the mongrel dog against supralethal doses of total-body radiation, but autologous marrow afforded some benefit at marginally lethal dosage levels. (auth)
- Research Organization:
- Sloan-Kettering Inst., New York; New York Veterans Administration Hospital
- NSA Number:
- NSA-13-020821
- OSTI ID:
- 4236334
- Journal Information:
- Journal of the National Cancer Institute (U.S.) Changed to JNCI, J. Natl. Cancer Inst., Journal Name: Journal of the National Cancer Institute (U.S.) Changed to JNCI, J. Natl. Cancer Inst. Vol. Vol: 23; ISSN JNCIA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
MODIFICATION OF X-RADIATION LETHALITY BY AUTOLOGOUS MARROW INFUSION IN DOGS
HOMOLOGOUS BONE MARROW TRANSPLANTATION IN DOGS RECEIVING X RADIATION PLUS URETHANE OR 6-MERCAPTOPURINE
Related Subjects
ANTIBIOTICS
BIOLOGY AND MEDICINE
BLOOD
BLOOD CIRCULATION
BODY
BONE MARROW
DIFFUSION
DOGS
DRUGS
EFFICIENCY
HORMONES
IMMUNITY
IRRADIATION
LETHAL DOSE
LEUCOCYTES
LYMPH SYSTEM
RADIATION DOSES
RADIATION INJURIES
RADIATION PROTECTION
SPLEEN
STANDARDS
STEROIDS
SURVIVAL TIME
SUSPENSIONS
TRANSPLANTS
VARIATIONS
X RADIATION