Chemical Protection for the Incorporation of Phosphorus-32 into Nucleic Acids of Lymphatic Cells against γ-Irradiation

Journal Article · Fri Mar 01 23:00:00 EST 1963 · Nature (London)

DOI:https://doi.org/10.1038/197914a0· OSTI ID:4727676

HONJO, ICHIJIRO; TCHOE, YUONG-TCHEL; TAKAMORI, YASUHIKO; AKABOSHI, MITSUHIKO

The protective effects of cysteamine and s,2-aminoethylisothiuronium (AET) for the incorporation of P³² by lymphatic cells were measured. Lymphatic cells were irradiated in vitro witli Co⁶⁰ gamma rays either immediately before or 5 to 10 min after the addition of cysteamine or AET in various concentrations. Desoxyribonucleic acid, ribonucleic acid, and organic acid-soluble phosphate were isolated and the specific activity of each fraction was determined as c/min/ mu g phosphorus. Results indicate that the threshold for the response of lymphatic cells to gamma radiation, measured by the incorporation of P³² into nucleic acids, is about 10 r. Finally, cysteamine was effective when present at the time of irradiation and AET failed to give protection for the incorporation of P³² into lymphatic cells.

• Research Organization: Osaka Univ.
• Sponsoring Organization: USDOE
• NSA Number: NSA-17-015833
• OSTI ID: 4727676
• Journal Information: Nature (London), Journal Name: Nature (London) Journal Issue: 4870 Vol. 197; ISSN 0028-0836
• Publisher: Nature Publishing Group
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AET
AMINES
BIOCHEMISTRY
BIOLOGY AND MEDICINE
COBALT 60
DNA
EFFICIENCY
ETHYL RADICALS
GAMMA RADIATION
GAMMA SOURCES
LYMPHOCYTES
MEA
MEASURED VALUES
METABOLISM
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHATES
PHOSPHORUS 32
QUANTITY RATIO
RADIATION DOSES
RADIATION PROTECTION
RADIOACTIVITY
RIBONUCLEIC ACID
SOLUBILITY
VARIATIONS