IN VITRO INCORPORATION OF $sup 32$P INTO NUCLEIC ACIDS OF LYMPHATIC CELLS. EFFECTS OF X-IRRADIATION AND SOME OTHER AGENTS
Lymphatic cells obtained from rabbit appendix were incubated with P/sup 32/ in Tyrode solution for 4 hours. The incorporation into DNA proceeded linearly while that into RNA tended to taper oft The specific activity ratio RNA/ DNA thus became much lower for the incorporation during 2 to 4 hours than during 0 to 2 hours of incubation. But if P/sup 32/ was added 2 hours after the start of incubation the RNA/DNA for 2 to 4 hours was as high as that for 0 to 2 hours with P/sup 32/ added at thc outset of incubation, suggesting that the rate of RNA synthesis might not change very much during thc incubation period of 4 hours. Several metabolic inhibitors affected the incorporation into nucleic acids in both radio-sensitive lymphatic cells and more radio-resistant Ehrlich ascites carcinoma cells incubated under similar conditions; the inhibition for DNA was sometimes much more severe than that for RNA. In contrast, effects of x irradiation (800 r) or hypertonic sucrose solution were markedly dissimilar between the two types of cells. In lymphatic cells, these agents produced a moderate inhibition of incorporation into both DNA and RNA, and the effect became conspicuous only from the second hour of incubation onward. In Ehrlich carcinoma cells the effect of these two agents was not remarkable and probably not specific to nucleic acids. Hypertonic sucrose solution curtailed the effectiveness of x irradiation, but dinitrophenol also had a similar effect. Whether x irradiation and hypertonic sucrose solution have something in common in their mode of action on lymphatic cells therefore remains to be determined. But under the present experimental conditions the effect of these two agents is neither immediate nor specific to DNA synthesis. (auth)
- Research Organization:
- Yamaguti Medical School, Ube, Japan
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-13-015004
- OSTI ID:
- 4243025
- Journal Information:
- Exptl. Cell Research, Journal Name: Exptl. Cell Research Vol. Vol: 17
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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Related Subjects
BIOCHEMISTRY
BIOLOGY AND MEDICINE
CANCER
CORROSION PROTECTION
DNA
DNP
EHRLICH ASCITES
INHIBITION
LYMPH SYSTEM
METABOLISM
NUCLEIC ACIDS
ORGANIC NITROGEN COMPOUNDS
PHENOLS
PHOSPHORUS 32
QUANTITY RATIO
RABBITS
RADIATION DOSES
RADIATION EFFECTS
RADIOCHEMISTRY
RADIOSENSITIVITY
RIBONUCLEIC ACID
SOLUTIONS
STERILIZATION
SUCROSE
SUGARS
TRACER TECHNIQUES
TUMORS
VARIATIONS
X RADIATION