Effects of X- and Ultra-violet Irradiation on the Incorporation of Phosphorus-32 into Isolated Nuclei of Rabbit Appendix
In previous studies it was observed that the removal of desoxyribonucleic acid from rabbit appendix nuclei isolated in sucrose severely impairs the incorporation of phosphorus-32 into desoxyribonucleic acid, ribonucleic acid, and organic acid-soluble phosphate. The lost capacity fcr incorporation can be restored by the addition of some polyanionic compounds, such as calf thymus desoxyribonucleate, yeast ribonucleate, or chondroitin sulfate. By treating the nuclei with pancreattc desoxyribonuclease in the presence of chondroitin sulfate, the bulk of desoxyribonucleic acid may be removed from the nucleus without appreciably affecting its capacity for incorporation. The effect of treatment with pancreatic desoxyribonuclease in the presence of chondroitin sulfate on the capacity of phosphorus-32 uptake of isolated nuclei is tabulated. ( A.C.)
- Research Organization:
- Yamaguti Medical School, Ube, Japan
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-13-013027
- OSTI ID:
- 4239049
- Journal Information:
- Nature (London), Journal Name: Nature (London) Journal Issue: 4671 Vol. 183; ISSN 0028-0836
- Publisher:
- Nature Publishing Group
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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