ELECTROPHORETIC STUDIES ON THE HEMOGLOBIN OF DOGS EXPOSED TO PENETRATING RADIATION

Journal Article · Mon Jan 01 04:00:00 EST 1962 · Probl. Lab. Radiobiol., L'vovsk. Gos. Univ.

OSTI ID:4694668

Sukhomlinov, B F; Merenov, V V; Semenchuk, N N

ABS>Studies were made on the electrophoretic mobility of hemoglobin in dogs exposed to x rays, and also the adsorption curves of oxyhemoglobin in the visible part of the spectrum. Acute radiation sickness was produced in dogs of 6 to 12 kg weight by a single exposure to a dose of 600 to 800 r from a distance of 1 m, at a rate of 14 r/min. Electrophoresis of the hemoglobin was carried out repeatedly in agar gel by the method of Monnier and Fischer. The adsorption curves of the hemoglobin solution were studied by means of a spectrophotometer, and the amino acid composition of the hemoglobin was established by chromatography. The hemoglobin was investigated at regular intervals until the animals' death. The results showed that, in healthy dogs, hemoglobin behaves as a homogeneous substance under electrophoresis and migrates at a uniform speed towards the cathode. After exposure to radiation, the electrophoretic mobility of hemoglobin decreases and the substance migrates more slowly if the radiation sickness produced has been severe. In those cases in which the change in the velocity of migration was less marked, the radiation sickness proved to be milder and more free of complications. The intensity of the changes varied in definite phases which coincided with the phases of radiation sickness. The adsorption curves of the oxyhemoglobin solution, on the other hand, were completely identical in both irradiated and control dogs. From these facts, it is concluded that radiation affects mainly the synthesis of the protein component of the hemoglobin molecule but does not affect the pigment hem. This conclusion could be confirmed by chromatographic analysis which showed significant changes in the amino acid structure of the hemoglobin molecule after exposure to radiation. (OTS)

• Research Organization: Originating Research Org. not identified
• NSA Number: NSA-17-030410
• OSTI ID: 4694668
• Journal Information: Probl. Lab. Radiobiol., L'vovsk. Gos. Univ., Journal Name: Probl. Lab. Radiobiol., L'vovsk. Gos. Univ. Vol. Vol: No. 1
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ADSORPTION
AMINO ACIDS
ATOMS
BIOCHEMISTRY
BIOLOGY AND MEDICINE
CATHODES
CHROMATOGRAPHY
COLLISIONS
COLLOIDS
DIAGRAMS
DIFFUSION
DOGS
EFFICIENCY
ELECTROPHORESIS
HEMOGLOBIN
INTERACTIONS
MANY BODY PROBLEM
MASS
MOLECULES
OXIDATION
PHOTOMETRY
PIGMENTS
PROTEINS
RADIATION DOSES
RADIATION EFFECTS
RADIATION SICKNESS
RARE GASES
REGENERATION
SOLUTIONS
SPECTRA
SPECTROMETERS
TEMPERATURE
VARIATIONS
X RADIATION