Monoclonal antibodies specific for sickle cell hemoglobin
Journal Article
·
· Hemoglobin; (United States)
Two mouse hybridoma cell lines were isolated which produce monoclonal antibodies that bind hemoglobin S. The mice were immunized with peptide-protein conjugates to stimulate a response to the amino terminal peptide of the beta chain of hemoglobin S, where the single amino acid difference between A and S occurs. Immunocharacterization of the antibodies shows that they bind specifically to the immunogen peptide and to hemoglobin S. The specificity for S is high enough that one AS cell in a mixture with a million AA cells is labeled by antibody, and such cells can be analyzed by flow cytometry. Immunoblotting of electrophoretic gels allows definitive identification of hemoglobin S as compared with other hemoglobins with similar electrophoretic mobility. 12 references, 4 figures.
- Research Organization:
- Lawerence Livermore National Lab., CA
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 6105446
- Journal Information:
- Hemoglobin; (United States), Journal Name: Hemoglobin; (United States) Vol. 9:4; ISSN HEMOD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANEMIAS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
CELL FLOW SYSTEMS
DISEASES
ELECTROPHORESIS
GLOBIN
HEMIC DISEASES
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYBRIDOMAS
IMMUNE REACTIONS
KINETICS
MAMMALS
MICE
MONOCLONAL ANTIBODIES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
PIGMENTS
PORPHYRINS
PROTEINS
REACTION KINETICS
RODENTS
SCREENING
SICKLE CELL ANEMIA
SYMPTOMS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANEMIAS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
CELL FLOW SYSTEMS
DISEASES
ELECTROPHORESIS
GLOBIN
HEMIC DISEASES
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYBRIDOMAS
IMMUNE REACTIONS
KINETICS
MAMMALS
MICE
MONOCLONAL ANTIBODIES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
PIGMENTS
PORPHYRINS
PROTEINS
REACTION KINETICS
RODENTS
SCREENING
SICKLE CELL ANEMIA
SYMPTOMS
VERTEBRATES