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Monoclonal antibodies to drosophila cytochrome P-450's

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6024060
; ;
Hybridomas producing monoclonal antibodies were prepared by the fusion of SP2/0 myeloma cells and spleen cells from a female BALB/c mouse immunized by cytochrome P-450-A and P-450-B purified from Drosophila Hikone-R (BG) microsomes. P-450-A and P-450-B are electrophoretically distinct subsets of Drosophila P-450. P-450-A is ubiquitous among strains tested, while P-450-B is present in only a few strains displaying unique enzyme activities and increased insecticide resistance. The Oregon-R strain contains only cytochromes P-450-A and is susceptible to insecticides. The authors Hikone-R (BG) strain expresses both cytochromes P-450-A and P-450-B and is insecticide resistant. Antibody producing hybridomas were detected in a solid-phase radioimmunoassay (RIA) by binding to Hikone-R (BG) or Oregon-R microsomes. Four independent hybridomas were identified as producing monoclonal antibodies that recognized proteins in the P-450 complex by immunoblot experiments. Three monoclonal antibodies recognized P-450-A proteins, while one monoclonal antibody bound predominantly P-450-B. This monoclonal antibody also recognized southern armyworm (Spodoptera eridania, Cramer) microsomal proteins.
Research Organization:
Univ. of Tennessee-Oak Ridge Graduate School of Biomedical Sciences
DOE Contract Number:
AC05-84OR21400
OSTI ID:
6024060
Report Number(s):
CONF-870644-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIBODIES
ARTHROPODS
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
DIPTERA
DROSOPHILA
ELECTROPHORESIS
ENZYME ACTIVITY
FLIES
FRUIT FLIES
HYBRIDOMAS
IMMUNOASSAY
IMMUNOLOGY
INSECTICIDES
INSECTS
INVERTEBRATES
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MICE
MICROSOMES
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
ORGANOIDS
PESTICIDES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
REACTION KINETICS
RODENTS
TRACER TECHNIQUES
VERTEBRATES