IN VITRO STUDIES OF THE MECHANISM OF ACTION OF CHEMICAL PROTECTIVE SUBSTANCES. PART I

Journal Article · Tue Jan 01 04:00:00 EST 1963 · Radiobiologiya (U.S.S.R.) For English translation see Radiobiology (Engl. Transl.)

OSTI ID:4682483

Ganassi, E E; Eidus, L Kh; Arifulina, R A

A systematic study of the protective action of substances of various types has been undertaken. Aqueous solutions of pepsin, 0.02 mg/ml (6 x 10/sup - 7/ M) at pH 4.5--4.6, were irradiated at 370 r/min, and in one case at 700 r/min, by a Co/sup 60/ or Cs/sup 137/ gamma source, in the presence of 10/sup -5/ to 10/sup -2/ M protective substance (AET, sodium nembutal, cystamine, sodium metabisulfite, BETA -alanine). The degree of inactivation was determined by measuring the proteolytic activity. The dose curve was in two parts, indicating two fractions, the first being considerably more rapidly inactivated than the second. The fraction of the slowly inactivated part depended on the concentration of the protectant. AET and sodium nembutal at 4.5 x 10/sup -4/ M concentration, cystamine at 3 x 10/sup -3/ M, sodium metabisulfite at 1.4 x 10/ sup -2/ M, and BETA -alanine at 4.3 x 10/sup -2/ M gave 10--80 percert protection. Analysis of the dose curves with differert concentrations of protectants indicated a protection mechanism that includes blocking of part of the surface of the protein by the protective substance from the approach of oxygen, with which an oxygen-type damage could occur. (TTT)

• Research Organization: Inst. of Biological Physics, Moscow
• NSA Number: NSA-17-031940
• OSTI ID: 4682483
• Journal Information: Radiobiologiya (U.S.S.R.) For English translation see Radiobiology (Engl. Transl.), Journal Name: Radiobiologiya (U.S.S.R.) For English translation see Radiobiology (Engl. Transl.) Vol. Vol: 3; ISSN RADOA
• Country of Publication: Country unknown/Code not available
• Language: Russian

Related Subjects

ACIDITY
AET
ALANINE
AMINES
AMINO ACIDS
ANESTHESIA
BARBITURIC ACID
BIOCHEMISTRY
BIOLOGY AND MEDICINE
CESIUM 137
CHEMICALS
COBALT 60
CYSTAMINE
CYSTEINE
DIAGRAMS
DRUGS
ENZYMES
GAMMA SOURCES
MEASURED VALUES
NEMBUTAL
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN
PEPSIN
PROTEINS
QUANTITY RATIO
RADIATION DOSES
RADIATION EFFECTS
RADIATION PROTECTION
SOLUTIONS
VARIATIONS
WATER