STUDIES ON THE GROWTH-INHIBITING AND RADIOPROTECTIVE EFFECT OF CYSTAMINE, CYSTEAMINE, AND AET ON MAMMALIAN CELLS IN TISSUE CULTURE

Journal Article · Sat Feb 01 04:00:00 EST 1964 · Radiation Res.

DOI:https://doi.org/10.2307/3571556· OSTI ID:4105742

Eker, P; Pihl, A

The growth-inhibiting and radioprotective effect of cystamine, cysteamine, and AET was studied in tissue culture of strain L mouse fibroblasts. When present in the growth medium during the 4-day test period, cystamine and cysteamine in concentrations above 0.9 x 10/sup -4/ M and 2 x 10/sup -4/ M, respectively, inhibited growth in monolayers. AET inhibited growth in concentrations exceeding 7 x 10/sup -4/ M. When the cells were suspended in saline and exposed to the sulfur compounds for 60 minutes only, concentrations of 2 x 10/sup -3/ M did not inhibit the subsequent growth in monolayers or in suspension culture. In the protection experiments the cells were suspended in Earle's salt solution and irradiated with x rays after preincubation with the test substances for 25 minutes. The subsequent growth in suspension culture was measured each 24 hours for 4 days. With cystamine and cysteamine in concentrations up to 2 x 10/sup -3/ M, no protection was found. With 2 x 10/sup - 3/ M AET significant protection was obtained against the growthinhibiting effect of 500 r of x rays. Sulfhydryl group titrations demonstrated that the mercaptoethylgranidine formed from AET was stable during the preincubation and exposure. Hence, the protective effect of AET cannot be attributed to oxygen depletion of the solution. Data are presented indicating that the failure of cysteamine to protect the L-cells is not due to lack of cellular uptake or to a rapid oxidation of the thiol to the inactive disulfide. The results indicate that different cell types may respond differently to the growth-inhibiting and radioprotective effect of the sulfur-containing agents. (auth)

• Research Organization: Norsk Hydro's Inst. for Cancer Research, Oslo
• Sponsoring Organization: USDOE
• NSA Number: NSA-18-011875
• OSTI ID: 4105742
• Journal Information: Radiation Res., Journal Name: Radiation Res. Vol. Vol: 21
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AET
ANIMAL CELLS
BIOLOGY AND MEDICINE
CYSTAMINE
MEA
MEG
MICE
ORGANIC SULFUR COMPOUNDS
OXIDATION
OXYGEN
QUANTITY RATIO
RADIATION DOSES
RADIATION EFFECTS
RADIATION PROTECTION
SALTS
SOLUTIONS
SUSPENSIONS
TISSUES
TOXICITY
X RADIATION