ON THE INTERACTION BETWEEN BILIRUBIN AND PLASMA ALBUMIN

Journal Article · Mon Jan 01 04:00:00 EST 1962 · Protides of the Biological Fluids, Proceedings of the Colloquium (Belgium)

OSTI ID:4680541

Barac, G; Beaumariage, M L; Onkelinx, C; Motay, C

Bilirubin solutions were x irradiated with doses up to 100,000 r in attempts to elucidate the nature of its interaction with serum albumin. It was expected that the reaction of radicals, formed during the radiolysis of water, with the pigment might provide some information on the groups involved in binding albumin. In aerated aqueous solutions free of proteins, sodium bilirubinate and bilirubin were oxidized by the action of x rays to form propentdiopent. This effect becomes marked at doses above 5000 r. In the presence of plasma proteins, the oxidation is considerably reduced. After 100,000 r, 84% of the original bilirubin could be recovered from a solution containing plasma, compared with 8% from an aqueous solution of bilirubin at pH 7.4. In addition to whole plasma, human albumin or its mercurial dimer also exerted a radioprotective effect on bilirubin. It is suggested that protection is afforded at the methane group of bilirubin and that the group is involved in its binding to albumin. (H.H.D.)

• Research Organization: Universite, Liege
• NSA Number: NSA-17-024867
• OSTI ID: 4680541
• Journal Information: Protides of the Biological Fluids, Proceedings of the Colloquium (Belgium), Journal Name: Protides of the Biological Fluids, Proceedings of the Colloquium (Belgium) Vol. Vol: 10; ISSN PBFPA
• Country of Publication: Country unknown/Code not available
• Language: French

Related Subjects

ACIDITY
ALBUMINS
BILIRUBIN
BIOLOGY AND MEDICINE
BLOOD PLASMA
FREE RADICALS
OXIDATION
PIGMENTS
RADIATION EFFECTS
RADIATION PROTECTION
RADIOCHEMISTRY
WATER
X RADIATION