STUDY OF THE FAST-NEUTRON EFFECT ON THE SPLEEN OF WHITE MICE BY A TISSUE- CULTURE METHOD

Journal Article · Tue Jan 01 04:00:00 EST 1963 · Radiobiologiya (U.S.S.R.) For English translation see Radiobiology (Engl. Transl.)

OSTI ID:4665712

Ivanitskaya, A F

The effect of a lethal irradiation dose, 600 rads, on the spleens of white mice was studied by means of tissue cultures. The reactor beam used for irradiation was a mixture of fast neutrons and gamma rays. The animals survived 3.1 days, and spleens were removed on the day of irradiation and 1, 2, and 3 days afterwards and were observed in the culture for 4 to 7 days. Lymphoid cells of the excised spleens of irradiated animals were characterized by shortening of their life to 3 to 4 days, decreased motility, appearance of binuclear forms; in the growth zones of spleens removed 3 days after irradiation there were single celis or groups. Granulocytes were very mobile, moving from the excised spleen to the medium; they disappeared after 4 days. Immature granulocytes were seen in spleens excised the day after irradiation but not in later excised ones. There were fewer migrating granulocytes in later excised ones. The granulocyte life decreased from 5 to 3 to 4 days. Stroma cells were not uniform and consisted of cells of the fibrotic type of capsule and trabecule, of the ordinary reticular stroma type, free cells of the macrophage type, and cells of the vascular endothelium. Polynuclear cells and mitoses were found regardless of the time of excision, mitoses reaching a maximum in the third to fifth day of culture but always being less than controls. Results are compared with those of many other workers. (21 references). (TTT)

• Research Organization: Severtsov Animal Morphology Inst., Moscow
• NSA Number: NSA-17-031946
• OSTI ID: 4665712
• Journal Information: Radiobiologiya (U.S.S.R.) For English translation see Radiobiology (Engl. Transl.), Journal Name: Radiobiologiya (U.S.S.R.) For English translation see Radiobiology (Engl. Transl.) Vol. Vol: 3; ISSN RADOA
• Country of Publication: Country unknown/Code not available
• Language: Russian

Related Subjects

AGE
ANIMAL CELLS
BIBLIOGRAPHY
BIOLOGY AND MEDICINE
BLOOD VESSELS
CYTOLOGY
FAST NEUTRONS
GAMMA RADIATION
LETHAL DOSE
LEUCOCYTES
LYMPH SYSTEM
MACROPHAGES
MICE
MITOSIS
NEUTRON BEAMS
PHYSIOLOGY
RADIATION DOSES
RADIATION EFFECTS
REACTORS
SPLEEN
SURGERY
SURVIVAL TIME
TISSUES
VARIATIONS