QUANTITATIVE STUDY ON INTERACTIONS OF CELLULAR GRAFTS IN MOUSE RADIATION CHIMAERAS

Journal Article · Tue Jan 01 04:00:00 EST 1963 · Folia Biologica (Prague) (Czechoslovakia)

OSTI ID:4654469

Lengerova, A

Lethally irradiated mice were given a curative dose of fetal liver cells of two different genotypes. By varying the conditions of administration of the two cell grafts, their relative capacity to repopulate the host bone marrow was made assessable. In the first experiment, changing the relative proportions of (CBA x T6T6)F/sub 1/ and (CBA x A)F/sub 1/ cells in the restorative inoculum from 8:2 to 2:8 did not markedly affect the proportions of these two cell types in the bone marrow of 200-day-old CBA chimeras, the majority of dividing cells in all four experimental groups being of (CBA x T8T6)F/sub 1/ origin. Coexistence in the chimeras of two cell populations differing antigenically from each other indicates graft-to-graft tolerance; under this condition an immune mechanism can scarcely be held responsible for the interactions of both cell populations and differential physiological capacity is to be inferred. In another experiment the result of competitive repopulation was more easily upset by varying the conditions by the start. The irradiated recipients were A strain mice, and the fetal liver cells were of ASW and CBA origin. The two restorative cell grafts were given either both intravenously or one of them intravenously and the other intraperitoneally or subcutaneously. Graft-to-graft tolerance was tested by challenging the survivors wtth both ASW and CBA skin grafts. If both types of cells were given intravenously, the CBA genotype appeared physiologically superior to ASW, for the results of skin-grafting pointed to the capacity of CBA cells to overgrow a double size inoculum of ASW origin. If the CBA cells were given by a non-intravenous route, the result of the competition was reversed in favor of the ASW genotype. The possible role of immune and nonimmune factors is discussed. (auth)

• Research Organization: Inst. of Experimental Biology and Genetics, Czechoslovak Academy of Sciences, Prague
• NSA Number: NSA-17-033675
• OSTI ID: 4654469
• Journal Information: Folia Biologica (Prague) (Czechoslovakia), Journal Name: Folia Biologica (Prague) (Czechoslovakia) Vol. Vol: 9; ISSN FOBLA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANIMAL CELLS
ANTIGENS
BIOLOGY AND MEDICINE
BONE MARROW
CHIMERAS
FETUSES
GENETICS
IMMUNITY
IRRADIATION
LETHAL DOSE
LIVER
MICE
RADIATION SICKNESS
REGENERATION
SKIN
TRANSPLANTS