USE OF INTERNAL SCINTILLATION STANDARDS IN HETEROGENEOUS COUNTING SYSTEMS

Journal Article · Tue Oct 01 04:00:00 EDT 1963 · Analytical Biochemistry (U.S.)

DOI:https://doi.org/10.1016/0003-2697(63)90160-X· OSTI ID:4619532

Bloom, B

f posystem (TGP) with those obtained from the hexose degradation procedure, the relative H/sup 3/ and C/sup 14/ efficiencies of the individual systems were evaluated using toluene-H/sup 3/ and toluene-C/sup 14/ as the internal scintillation standards (ISS). Three samples of radioactive potassium gluconate were prepared from glucose-6-H/sup 3/, glucose-8-C/sup 14/, and glucose, C/sup 14/ by the method of Moore and Link. The salts were ground; suspended in toluene containing TGP, PPO, and POPOP; and assayed for radioactivity. Samples of three radioactive potassium gluconates were also converted to gluconic acid and the aqueous solutions reduced to thick syrups, which were then taken up in Hyamine. Counting was done in PPO and POPOP. Following counting of the samples, 20 mu l of toluene-H/sup 3/ and 20 mu l of toluene-C/sup 14/ were added to the sample to determine the relative efficiencies of the two counting systems. Data based on the activities of the internal scintillation standards indicate that the TGP system is the more efficient of the two systems with respect to both H/sup 3/ and C/sup 14/. However, inspection of the data obtained with the radioactive potassium gluconate and gluconic acid samples indicates that the Hyamine system is more efficient for both H/sup 3/ and C/sup 14/. (H.M.G.)

• Research Organization: National Inst. of Arthritis and Metabolic Diseases, Bethesda, Md.
• Sponsoring Organization: USDOE
• NSA Number: NSA-17-040499
• OSTI ID: 4619532
• Journal Information: Analytical Biochemistry (U.S.), Journal Name: Analytical Biochemistry (U.S.) Vol. Vol: 6; ISSN ANBCA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AMINES
BIOCHEMISTRY
BIOLOGY AND MEDICINE
CARBOHYDRATES
CARBON 14
CHEMICAL REACTIONS
EFFICIENCY
GLUCONIC ACID
GLUCOSE
HYAMINE
LABELLED COMPOUNDS
METABOLISM
ORGANIC ACIDS
ORGANIC NITROGEN COMPOUNDS
PERFORMANCE
PHOSPHORS
POPOP
POTASSIUM COMPOUNDS
PREPARATION
PROTEINS
RADIOACTIVITY
REACTION KINETICS
SAMPLING
SCINTILLATION COUNTERS
SOLUTIONS
STANDARDS
SUGARS
TOLUENE
TRACER TECHNIQUES
TRITIUM
WATER