Mutagenesis in cultured mammalian cells
Journal Article
·
· Stud. Biophys., v. 36-37, pp. 139-147
OSTI ID:4437941
From international conference on the bases of the biological effects of ultraviolet radiation; Brno, Czechoslovakia (2 Oct 1972). Variants of cultured mammalian cells may be obtained either by establishing cell lines from individuals with recognized metabolic disorders or by the selection of new phenotypes in existing cell lines. A range of drug-resistant or auxotrophlc lines was obtained by selection from a number of different species. The induction of variant clones requires that selective techniques are available. The 8-azaguanine resistance in Chinese hamster V79 cells satisfies this requirement. This system may be used to detect mutation induction by the photosensitlzing reaction on cells between 8-methoxypsoralen and 360 nm uv light. The availability of two uv-sensitive lines derived from the V79 cell line makes it possible to test the more general application of the repair model of mutagenesis in bacteria to mammalian cells. (auth)
- Research Organization:
- Univ. of Sussex, Brighton, Eng.
- NSA Number:
- NSA-29-000542
- OSTI ID:
- 4437941
- Journal Information:
- Stud. Biophys., v. 36-37, pp. 139-147, Journal Name: Stud. Biophys., v. 36-37, pp. 139-147; ISSN STBIB
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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